Cytogenetics lab

Contact the Cytogenetics lab

Phone: 7825 3237

Email: SCHN-CHW-CytogenLab@health.nsw.gov.au

Postal address: The Children's Hospital at Westmead, Locked Bag 4001, Westmead 2145

The Cytogenetics laboratory is service within the Sydney Genome Diagnostics (SGD) services based at The Children's Hospital at Westmead (CHW). We provide a NATA accredited service for the diagnosis of a wide range of constitutional (blood), prenatal and acquired (leukaemia, lymphoma and tumour) chromosome disorders.

We perform G-band karyotyping, fluorescence in-situ hybridsation (FISH), CGH and SNP Chromosome Microarray [CMA], quantitative fluorescent-PCR prenatal aneuploidy screening (QF – PCR) and RT-PCR testing (soft-tissue tumour biopsy specimens only).

These services are extended to all states and forms part of both the CHW Division of Laboratory Services and the Western Sydney Genetics Program.

Specimen collection

Test RequiredSample TypeSpecimen Requirements
Chromosome microarrayPeripheral Blood3-10mL collected in purple top (EDTA) tube
 

DNA

Amniotic Fluid *

Chorionic Villi *

Bone Marrow

Solid Tumour

Minimum of 1.0μg of DNA (concentration between 100 and 500 ng/μL)

15-20mL collected in sterile transport tube

15-40mg collected in sterile transport tube with tissue culture media

2-4mL collected in either Lithium Heparin or EDTA

1mm3 piece of tissue or core [needle] biopsy in sterile saline.

G-band karyotypePeripheral Blood2-10mL collected in green top lithium heparin tube
 Bone Marrow2-4mL collected in green top lithium heparin tube
 Solid Tumour0.5cm3 in transport media
Chromosome Instability Testing 

Test is no longer offered by The Children’s Hospital at Westmead

Test is now performed by Pathology Queensland

FISHPeripheral Blood2-10mL collected in green top lithium heparin tube
 Touch Imprints3-4 slides in 95% ethanol
 FACS Sorted Cells100,000-200,000 cells in PBS
 

Fixed cell suspension

Formalin Fixed Paraffin Embedded Tissue

3-4 + charged slides with tissue cut at 4µm and an H&E slide indicating the region of interest (please contact the laboratory for details)
 QF-PCRAmniotic Fluid *15-20mL collected in sterile transport tube
 Chorionic Villi *15-40mg collected in sterile transport tube with tissue culture media
RT-PCRTumour0.5cm³ snap frozen in liquid nitrogen
Cell Culture & Cryopreservation Facility Contact Biochemical Genetics: (02) 9845 3654
DNA Storage Contact Molecular Genetics: (02) 9845 3244

Senior staff

Head of Department (Principal Scientist): Mr Dale Wright

B.App.Sc, MSc, FHGSA (Cytogenetics). FFSc RCPA

Phone: 7825 3221

Email: Dale.Wright@health.nsw.gov.au

 

Deputy Head of Department [2IC] (Senior Scientist): Mr Luke St. Heaps

BSc, MSc(Med)

Phone: 7825 3220

Email: Luke.Stheaps@health.nsw.gov.au

 

Chromosome Microarray/RT-PCR (Senior Scientist): Mr Artur Darmanian

BSc, MSc

Phone: 7825 3228

Email: Artur.Darmanian@health.nsw.gov.au

 

Oncology Microarray/Oncology Cytogenetics (Senior Scientist): Ms Dorothy Hung

B.App.Sc, MSc, FHGSA (Cytogenetics). FFSc RCPA

Phone: 7825 3223

Email: Dorothy.Hung@health.nsw.gov.au

 

Oncology and Blood Cytogenetics (Senior Scientist): Mr Praveen Sharma

B.Sc, MScM, MHGSA (Cytogenetics)

Phone: 7825 3224

Email: Praveen.Sharma@health.nsw.gov.au

 

Prenatal Microarray/QF-PCR (Senior Scientist): Mr Con Ngo

B.Sc. FHGSA (Cytogenetics)

Phone: 7825 3231

Email: Con.Ngo@health.nsw.gov.au

Last updated Wednesday 5th June 2024